ABSTRACT
We introduce a pragmatic approach towards the corrected Base Excess (BE) by including the large variability of the apparent dissociation constant pK' in non-logarithmic form in Henderson-Hasselbach bicarbonate ion equilibria thereby resulting in a significant correction both in calculated bicarbonate ion concentration and BE at 37 degrees C.
Subject(s)
Acid-Base Equilibrium , Animals , Bicarbonates/analysis , Blood Gas Analysis/methods , Humans , Reproducibility of ResultsABSTRACT
We introduce computed value of the corrected Strong Ion Difference (SID) by including the large variability of the apparent dissociation constant pK' in non-logarithmic form on SID in Henderson-Hasselbach bicarbonate ion aqueous equilibria thereby resulting in a significant correction of up to 27% in SID. We further introduce a new concept of Strong Ion Difference Excess (SIDE) as the change in SID from the reference value at pH = 7.4, pCO2 = 5.33 Kpa (or 40 torr). The SIDE is a particularly useful quick measure when one can rule out the effects of hemoglobin, weak proteins and unidentified components for human blood plasma.
Subject(s)
Acid-Base Equilibrium , Bicarbonates/blood , Carbon Dioxide/blood , Humans , Models, BiologicalABSTRACT
We study the shapes and biochemical characteristics of human red blood cells using a unified biochemical and continuum mechanical model. In particular, we model the crenated, echinocytic shapes and show how they may shift due to changes in the pH and various amphipaths affecting the osmotic pressure by also utilizing pressure as an independent variable. In contrast to earlier works which advocate that biochemical factors may be attributable to mechanical control parameters, cytoskeletal elastic constants and effective relaxed bilayer area difference of outer plasma membrane and inner protein-based membrane skeleton, our unified model agrees well with Band 3 diffusion experimental root mean square distance data.